Journal: International Journal of Molecular Medicine

Article Title: Macrophage SH3 domain-containing GRB2-like protein B1 is required for Rab7-mediated mitophagy in inflammation during sea water drowning acute lung injury

doi: 10.3892/ijmm.2026.5809

Figure Lengend Snippet: SH3GLB1 deficiency protects against inflammation in ALI. (A) Gene expression of SH3GLB1 high AMs in cohort 1 (n=3) represented in a volcano plot, with the fold change (log2) expressed as the level in sham versus sea water-ALI subjects on the x-axis and the P-value on the y-axis (log10). Significantly (P<0.05) regulated genes are marked in colors: red indicates upregulated genes and blue indicates downregulated genes in ALI. (B) Representative GO analysis of SH3GLB1 high AMs. (C) Representative KEGG analysis of SH3GLB1 high AMs. (D) Representative hematoxylin and eosin staining of lung tissues (scale bars, 200 µ m). (E) Representative severity scores of lung injury (n=3). Representative ELISA results showing the levels of inflammatory factors, including (F) TNFα, (G) IL-6 and (H) IL-1β, in the BALF (n=6). (I-K) Representative ELISA analysis of the levels of inflammatory factors, including (I) IL-1β, (J) IL-6 and (K) TNFα, in the serum (n=6). (L) Representative western blots of SH3GLB1 protein levels in NC or SH3GLB1-KD BMDMs. (M) Relative SH3GLB1 mRNA levels in (NC or SH3GLB1-KD BMDMs. n=6/group. Representative ELISA analysis of the levels of inflammatory factors, including IL-1β, IL-6 and TNFα, in the supernatants of cells treated with (N) LPS or (O) seawater, (n=3).The data are presented as the means ± SEMs; P<0.05 was considered to indicate statistical significance. SH3GLB1, SH3 domain-containing GRB2-like protein B1; AMs, alveolar macrophages, ALI, acute lung injury; GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes; BALF, bronchoalveolar lavage fluid; BMDMs, bone marrow-derived macrophages; KD, knockdown; WT, wild-type; KO, knockout; NC, negative control; LPS, lipopolysaccharide.

Article Snippet: A total of 20 SH3GLB1-deficient mice on a C57BL/6J background and 20 of their wild-type littermates were produced by Shanghai Model Organisms Center.

Techniques: Gene Expression, Staining, Enzyme-linked Immunosorbent Assay, Western Blot, Derivative Assay, Knockdown, Knock-Out, Negative Control

Journal: International Journal of Molecular Medicine

Article Title: Macrophage SH3 domain-containing GRB2-like protein B1 is required for Rab7-mediated mitophagy in inflammation during sea water drowning acute lung injury

doi: 10.3892/ijmm.2026.5809

Figure Lengend Snippet: Restoration of SH3GLB1 expression provokes ALI. (A) Representative hematoxylin and eosin staining of lung tissues (scale bars, 100 µ m). (B) Representative severity scores of lung injury (n=3). Representative ELISA results showing the levels of inflammatory factors, including (C) TNFα, (D) IL-1β and (E) IL-6, in the BALF (n=6). Representative ELISA analysis of the levels of inflammatory factors, including (F) IL-1β, (G) IL-6 and (H) TNFα, in the serum (n=6). (I) Western blots of SH3GLB1 expression. (J) Relative SH3GLB1 mRNA levels in vectoror SH3GLB1-overexpressing BMDMs. n=6/group. Representative ELISA analysis of the levels of inflammatory factors, including IL-1β, IL-6 and TNFα, in the supernatants of cells treated with (K) LPS or (L) sea water (n=3).The data are presented as the means ± SEMs; P<0.05 was considered to indicate statistical significance. SH3GLB1, SH3 domain-containing GRB2-like protein B1; ALI, acute lung injury; BMDMs, bone marrow-derived macrophages; WT, wild-type; OV; overexpression.

Article Snippet: A total of 20 SH3GLB1-deficient mice on a C57BL/6J background and 20 of their wild-type littermates were produced by Shanghai Model Organisms Center.

Techniques: Expressing, Staining, Enzyme-linked Immunosorbent Assay, Western Blot, Derivative Assay, Over Expression

Journal: Biomaterial Investigations in Dentistry

Article Title: Acrylic-based occlusal device materials – the influence of manufacturing techniques on material properties and the propensity for biofilm formation

doi: 10.2340/biid.v13.45909

Figure Lengend Snippet: Quantification of S. mutans biofilm formation on surfaces of materials from the different workflows. The 24-hour bioreactor approach (A) and the 72-hour culture plate approach (B). Data are presented as mean and SD (error bars). Asterisk (*) indicates a statistically significant difference ( p < 0.05).

Article Snippet: In both experiments, the wild‐type Streptococcus mutans UA159 strain (ATCC 700610) was used as the inoculum.

Techniques: